1966. 1969. Fed. Clinical signs associated with the virus may include reduced production and litter sizes, as well as retarded growth of young within breeding colonies.
Linkage analysis using somatic cell hybrids. Virology 27: 115–130.Neff, J. M., and J. F. Enders. Abstract. Pharmacol. Proc. Immunoglobulin A anti-Sendai virus HN protein monoclonal antibodies, generated via a mucosal immunization protocol, were shown to neutralize virus in vitro and, when passively administered to the mouse respiratory tract, to protect against Sendai virus in vivo. 1965.
Nature (London) 225: 159–164.Koprowski, H.. 1971. Hybrid cells derived from mouse and man: Artificial heterokaryons of mammalian cells from different species. 127: 260–267.Roberts, J. J., and G. P. Warwick.
Cell Res. Bioz Stars score: 95/100, based on 27 PubMed citations. HNAFYKD, with SYWST replaced with the analogous sequence of measles virus (MeV) H (AFYKD), is not incorporated in virus particles produced by LLCMK2 cells, although it is normally expressed at the plasma membrane. Biken J. Exp. ),Watkins, J. F.. 1971. We propose that HN incorporation in virus particles requires first neutralization by HN of a putative inhibitor of infectious particle formation.We use cookies to help provide and enhance our service and tailor content and ads. Our CytoTune-iPS 2.0 Sendai Reprogramming Kits support cutting-edge basic research, and our CTS CytoTune 2.1 Sendai Reprogramming Kit facilitates clinical and translational research.
Unlike Sendai virus-induced disease in mice, an asymptomatic and self-limiting disease is usually induced by Sendai virus in rats. Int. Somatic cell fusion and hybridization. 1970. In Vitro 6: 411–426.Rao, P. N. and R. T. Johnson. Hum. 1967. 30: 930–934.Okada, Y.. 1969. A detailed protocol for the growth of Sendai virus in embryonated hen's eggs and for the preparation of inactivated Sendai virus for use in cell fusion experiments is described. 26: 98–107.Harris, H., and J. F. Watkins.
Unlike HNSYWST, HNAFYKD is not internalized to late endosomes, raising the possibility that HN internalization is required for uptake into virus particles. Nature (London) 205: 640–646.Ruddle, F. H.. 1970. Exp. Proc. In: P. F. Kruse and M. K. Patterson (Eds. Soc.
Microscopic observation of giant polynuclear cell formation. Of these, we here describe a protocol for Sendai virus plaque assay to provide PFU using LLC-MK2 cells (a rhesus monkey kidney cell lines) and Guinea pig red blood cells.
Biol. Sendai virus (SeV) HN protein is dispensable for virus particle production. 1968.
In: K. Maramorosch and H. Koprowski (Eds. 12: 1441–1442.Cassals, J. Subscription will auto renew annually.Over 10 million scientific documents at your fingertips Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis. Cell fusion and virus rescue. 1970. Giles, R.E., Ruddle, F.H. I. Curr. You can also search for this author in
Immunological techniques for animals viruses. 1963. HN incorporation into virions strictly depends on a cytoplasmic domain SYWST motif. Microbiol.
Symp. Factors in fusion by HVJ. However, when co-expressed with HNAFYKD these MeV-H chimera induced HNAFYKD uptake into virions showing that internalization is not a prerequisite for HN uptake into particles. Mammalian somatic cell hybrids and their susceptibility to viral infection. Sendai Virus, supplied by ATCC, used in various techniques. Home > Search Results > ATCC > sendai virus. Utilization of somatic cells for genetic analysis: possibilities and problems.
HNAFYKD, with SYWST replaced with the analogous sequence of measles virus (MeV) H (AFYKD), is not incorporated in virus particles produced by LLCMK2 cells, although it is normally expressed at the plasma membrane. By continuing you agree to the Copyright © 2020 Elsevier B.V. or its licensors or contributors. 30: 914–920.Green, H., R. Wang, C. Basilico, R. Pollack, T. Kusano, and J. Salas. Cell Res. Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta-propiolactone inactivated Sendai virus. A quantitative comparison of formation of spontaneous and virus produced viable hybrids. Proc. Cell Biol. 1961.
A detailed protocol for the growth of Sendai virus in embryonated hen's eggs and for the preparation of inactivated Sendai virus for use in cell fusion experiments is described.Immediate online access to all issues from 2019. Various mosaic MeV-H containing increasing amounts of the SeV-HN all failed to be taken up in SeV virions. U.S.A. 62: 852–859.Steplewski, Z., and H. Koprowski.
J. Acad. Cell Biol. PRODUCTION OF SENDAI VIRUS FOR CELL FUSION RICHARD E. GILES AND FRANK H. RUDDLE Yale University, Kline Biology Tower, New Haven, Connecticut 06520 SUMMARY A detailed protocol for the growth of Sendai virus in embryonated hen's eggs and for the preparation of inactivated Sendai virus for use in cell fusion experiments is de-scribed. Fed. Biochem. ),Giles, R. E., and F. H. Ruddle.
Immediate online access to all issues from 2019.
In: H. Busch (Ed.
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