Complete reprogramming of iPS cell colonies is confirmed by expression of pluripotency markers and appropriate colony morphology. 8.
Successful generation of iPS cells from human foreskin fibroblasts after infection with single-vector Human STEMCCA Cre-Excisable Lentivirus (Cat.
STEMCCA Vector Advantages: Efficient: uses a single vector with four transcription factors rather than … The StemLight™ iPS cell Reprogramming Antibody Kit contains a panel of antibodies for the detection of various proteins, combinations of which have been used to reprogram somatic cells to Induced Pluripotent Stem (iPS) cells.
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SCR545), as indicated by expression of characteristic pluripotency markers. Not for use in diagnostic procedures.
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1-2 See More The CytoTune™-iPS 2.0 Sendai Reprogramming Kit typically exhibits greater than 2-fold efficiency over the original kit, for both fibroblast and blood reprogramming. reprogramming factor genes by exploiting the cytoplasmic nature of SeV and the functional temperature sensitivity mutations introduced into the key viral proteins. endstream
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Derive cell lines from diverse genetic backgrounds directly starting from a myriad of cell types, including PBMCs and T-cells. No. Simplicon™ RNA Reprogramming Kit (OKSG) EMD Millipore’s Simplicon™ RNA Reprogramming Kit is a safe and efficient method to generate integration free, virus-free human iPS cell using a single transfection step of self-replicating synthetic RNA for Oct4, Klf4, Sox2 and Glis1.
(BJ and CD34+ cells, respectively). Kit Contents SCR545), as indicated by expression of characteristic pluripotency markers. Store at -5 to -20°C. For Research Use Only. Efficiencies greater than 1% have been obtained with both fibroblasts and blood cells. Other reprogramming methods, such as lentivirus and retrovirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results. One kit of four tubes is enough for 2 wells of a 6-well plate (5 x 105cells/well) at MOI = 3. The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences driven by the EF-1α constitutive promoter The CytoTuneiPS Reprogramming Kit contains four SeV™- -based reprogramming vectors, each capable of expressing one of the four Yamanaka factors (i.e., Oct4, Sox2, Klf4, and c- Myc) and are optimized for generating iPSCs from human somatic cells. %PDF-1.6
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STEMCCA™ Lentivirus Reprogramming Kits Figure: Successful generation of iPS cells from human foreskin fibroblasts after infection with single-vector Human STEMCCA Cre-Excisable Lentivirus (Cat. Induced pluripotent stem (iPS) cells are a type of pluripotent stem cell (PSC) that can be obtained by reprogramming somatic cells through artificial expression of key transcription factors under specific culture conditions. Our CytoTune-iPS 2.0 Sendai Reprogramming Kits support cutting-edge basic research, and our CTS CytoTune 2.1 Sendai Reprogramming Kit facilitates clinical and translational research. The Human iPS Cell Reprogramming Retrovirus Kit offers such opportunity to generate iPSCs from various tissues and cell types. A validated episomal vector reprogramming kit that produces footprint-free iPS cells that can be used for all stages of your pre-clinical research. The reprogramming vectors in this kit have been engineered to increase biological and environmental safety (see No. The Epi5™ Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. h��UmLSg>�-\��-���Q�sݤZ?��_�qJ\\��ִx���,�����sq�:*��
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For use with associated StemRNA 3 rd Gen Reprogramming Kit protocols for generation of iPS cells. Use the links below to find out how the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit can revolutionize your cell reprogramming. Nuclei are stained with DAPI (blue). Not for use in diagnostic procedures. �d`b�T�e`�!��e�[� oBq
Retrovirus iPSC Reprogramming Kit: #RF101 Product Description Human induced pluripotent stem cells (iPSCs) can be derived from somatic cells through a reprogramming process driven by ectopic expression of a defined set of reprogramming factors: Oct4, Sox2, Klf4 and c-Myc. h�bbd``b`� �)@��H0ǃ�K"�U"V��+ %�A+�C��$�A�8 ����z@��@�/
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